Fluorescence Recovery After Photobleaching

Intelligent Imaging Innovations' Fluorescence Recovery After Photobleaching (FRAP) system offers for the first time the ability to do high-speed computer controlled FRAP experiments on a wide-field or spinning disk confocal microscope. This system, developed by Photonic Instruments and 3i, is the only system of its kind currently available. Fully integrated with SlideBook™, the system allows the experimenter to define freeform FRAP, photo-ablation or photo-uncaging regions. Once the regions are defined, the experimenter can perform the photobleaching at the touch of a button mid-experiment.

The laser system itself can be retrofitted onto most fluorescence microscopes. It consists of a fiber optically pumped tunable dye laser, computer controlled steering optics, power control and SlideBook™ software.

Features:

• Simultaneous laser / fluorescence illumination
• Fiber optic delivery system provides consistent safety, performance and alignment even if components are moved
• Change a dye cell and tune your system within the visible spectrum. Molecularly Selective Ablation / Illumination is possible with one laser system
• Steering optics ensure fast, efforless and accurate beam alignment to a crosshair, fiduciary mark or positioning to an experimental target
• Z axis telescope allows the user to control the plane of ablation
• Variable attenuator slide provides incremental control of energy

Performance:

• Energy up to 50µ Joules
• Pulse length 2-6 nano seconds
• Near diffraction limited spot size
• Tunable from 375-900nm
• Reusable dye cell provides 100,000 pulses from ~5ml disposable dye
• Nitrogen laser provides 20,000,000 pulses from replaceable plasma cartridge