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Precise. Fast. Marianas SDC™ provides image acquisition and analysis at breathtaking speed while sacrificing none of the precision required by quantitative microscopy. A sample of the key capabilities offered by Marianas SDC™ includes:

• 2D/4D Streaming acquisition
• Multi-Camera Simultaneous acquisition
• Yokogawa CSU-X provides real-time optical sectioning at up to 2000 fps
• XY positioning via linear encoders
• Z positioning via harmonic/piezoelectric control
• TTL module provides fully-closed and fully-integrated synchronization of event triggering and acquisition to simplify and quicken capture

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Quantitative Excellence. Marianas SDC™ integrates state-of-the-art optics and software to deliver images and datasets of unparalleled quality and integrity. Here's how:

• Zeiss optics provide state-of-the-art transmission and resolution characteristics
• Yokogawa CSU-X technology optimizes brightness and signal-to-noise ratio for confocal imaging
• Motorized Spherical Aberration Correction (SAC) device remedies the most serious monochromatic defect in research microscopy
• Scientific-grade EMCCD detector is engineered to maximize sensitivity, speed, and linearity while suppressing read-noise and dark current.
• SlideBook™ 5 suite provides comprehensive statistical analysis capabilities with a user-friendly interface

Maximum Sample Viability. Marianas SDC™ takes the steps necessary to minimize observational impact on the sample, yielding maximum experimental significance. Several of the elements vital to this effort involve:

• Dynamic environmental control of temperature, humidity, and CO₂ levels
• TTL module provides fully-closed and fully-integrated synchronization of event triggering and acquisition to minimize sample exposure to excitation wavelengths
• Yokogawa CSU-X scanning disk and optical technology results in ultra-low sample photobleaching and phototoxicity

Comparison: 10 micron liver tissue section stained with hematoxolin collected.

Unbounded Acquisition. Marianas SDC™ eliminates the traditional limitations associated with quantitative microscopy capture. As a result, the experiment is dictated by the investigator, and not by the acquisition system.

• Motorized XYZ positioning allows for multiplex capture and analysis
• SlideBook™ 5 provides periodic autofocus correction during time-lapse acquisition and works in conjunction with Definite Focus, an infrared-based autofocus solution developed by Zeiss
• SlideBook™ 5 eliminates memory constraints with spool-to-disk capture capabilities

Customizable. Versatile. Expandable. Marianas SDC™ offers near-infinite levels of optimization to address a wide range of scientific applications:

• Optical components are tailored specifically to the individual requirements of the researcher
• LaserStack is a highly customizable launch for various laser applications.¹
• 300W Xenon light source provides spectrally flat critical illumination for a wealth of epi-fluorescent applications
• Marianas SDC™ is modular and solutions are offered for:
  • TIRF - Total Internal Reflection Fluorescence
  • FRAP - Fluorescence Recovery After Photobleaching
  • PA - Photoactivation
  • FLIM - Fluorescence Lifetime Imaging
  • 2-Photon Imaging

1. Complies with FDA performance standards for laser products except for deviations pursuant to Laser Notice No. 50, dated July 26, 2001