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Creating Bead Slides

Bead Slides are a useful tool in determining Point Spread Functions for optics.

The following supplies are needed:

  • Glass Slides
  • Clear Nail Polish
  • Cover Slip
  • Pipetteman™
  • Molecular Probes PS-Speck™ Microscope Point Source Kit *blue, green, orange, and deep red fluorescent beads*
  • P-7220

Bead slide directions:

PS-Speck™ beads

  1. Dilute red, green, blue and orange beads 1:500 with DI water. Keep bead colors separate.
  2. Add 50 ul of diluted beads to cover slip and allow to dry, one coverslip per color.
  3. Add ~50 ul of mounting medium to dried beads.
  4. Invert coverslip onto slide.
  5. Wipe excess mounting medium from edges of cover glass and seal with clear nail polish, brushing in a single direction and overlapping the cover glass and slide.
  6. Let dry and keep out of light as much as possible.
  7. Store in freezer when possible.