Versatile live cell light sheet microscope
Rapid 3D imaging with isotropic resolution and low phototoxicity
The Marianas LightSheet™ merges the low phototoxicity and large specimen handling of dual inverted selective plane illumination microscopy (diSPIM) with the power and flexibility of a live-cell microscope system.
diSPIM employs two orthogonal objectives positioned at 45° above the specimen plane. By alternating between the objectives for imaging and excitation, diSPIM captures two volumes that are fused and deconvolved to achieve isotropic resolution.
Selective plane illumination microscopy (SPIM) uses a thin sheet of light to illuminate only the plane of interest, reducing phototoxicity by drastically cutting total light dose and allowing for prolonged specimen imaging.
The light sheet can be swept through the sample at high speed while a piezo moves the imaging objective to capture up to 3 volumes per second. Larger samples can be moved beneath the objectives.
Live Dictyostelium cells with actin label
Unprecedented light sheet specimen accessibility and numerous imaging modes
The Marianas LightSheet™ allows for horizontal specimen mounting for conventional sample preparations such as coverslips and Petri dishes and provides a platform for custom-designed chambers. Embedding the specimen in gel is optional.
The open sample chamber accommodates specimens of various sizes. The inverted microscope enables easy sample location and positioning.
A light sheet from a scanned Gaussian beam enables rapid 3D imaging of samples ranging from single cells to organisms over the course of hours to days. The inverted microscope enables long term live cell experiments with available perfusion and incubator for full environmental control.
Four different scan modes allow for experimental flexibility whether your focus is on imaging speed, sample size or reduced light dose.
Drosophila embryo with DAPI staining of DNA.
HeLa cells with fluorescently labeled Actin and DNA.
A system tailored to your imaging and analysis needs
The Marianas LightSheet™ is a powerful live-cell imaging workstation that can be customized and combined with spinning disk confocal microscope, TIRF, FLIM, Laser Ablation, Photomanipulation and Digital Holography. SlideBook™ software seamlessly manages acquisition, alignment, registration, deconvolution and rendering.
The ideal solution for cell viability
CELL VIABILITY
Peak light intensity is greatest in point scanning methods. Total light dose increases in methods that illuminate the entirety of the cell.
LIGHT DOSE / PHOTOTOXICITY
SPIM is ideal for long-term live cell imaging due to the low light intensity
compared to confocal and laser scanning systems.
SlideBook
Fully integrated software solution
Capture, register, deconvolve and analyze in one software
Integrated hardware and software control creates a seamless user experience with complete comprehensive control of acquisition and rendering.
• Software assisted alignment
• High-speed registration, deconvolution and 3D reconstruction
• Custom scripting and MATLAB® integration
Zebrafish larva three days past fertilization expressing LifeAct GFP, courtesy of Dr. Cody Smith, University of Notre Dame, US
Specifications
OBJECTIVES
10X water immersion, 0.3 NA, 3.5mm WD
20X water immersion, 0.5 NA, 3.5mm WD
40X water immersion, 0.8 NA, 3.5mm WD
16X multi-immersion RI 1.33-1.56, 0.4 NA, 12mm WD, for Cleared Specimens
24X multi-immersion RI 1.33-1.56, 0.7 NA, 10mm WD, for Cleared Specimens
OBJECTIVE TRAVEL
150µm piezo translators
SPECIMEN MOUNTING
Horizontal stainless steel chamber
50mm x 24mm coverglass standard
95mm Petri dish
Custom chambers available upon request
CAMERA
2048×2048 16-bit sCMOS 100fps
Liquid cooling
LASER LINES
405nm, 445nm, 488nm, 515nm, 561nm, 594nm, 640nm
ENVIRONMENTAL CONTROL
Available perfusion system and full chamber incubator
COMPUTER
Dual 12-Core Xeon 2.3GHz CPUs
128GB RAM, 4GB GPU, 512GB solid state drive
2TB SSD RAID-10 drive array for data acquisition
10Gb Ethernet with optional multi-mode and single-mode fiberoptic connectivity