Advanced Multimodal Microscopy

Systems and Solutions

3i designs and manufactures technologies for living cell, live cell, and intravital fluorescence microscopy including superresolution, computer-generated holography, spinning disk confocal, multi-photon and lightsheet. SlideBook software manages everything from instrument control to image capture, processing and data analysis.

HeLa cells transfected with GFP-Lifeact (488nm) to visualize actin dynamics and stained with DiL (561nm red)(Thermo) to visualize membrane dynamics.

Courtesy James Springfield, Institute for Molecular Bioscience (IMB) at the University of Queensland

Cell Division

Captured using 3i Marianas and CSU-W1 SoRa


Courtesy of Dr. S. Okabe & Dr. S. Tanaka, Department of Cellular Neurobiology, University of Tokyo

Two- and three-photon microscopy of the corpus callosum in a 6-week old transgenic mouse which specifically labels oligodendrocytes (Mobp-EGFP).

Images courtesy of Drs. Ethan Hughes, Emily Gibson, and Diego Restrepo at the University of Colorado Anschutz Medical Campus.

Movie of neutrophil and platelets in the inflamed cremasteric venules of a WT mouse, 3h after local injection of TNFa. Cells are labeled by injection of low amounts of antibodies anti-Ly6G conjugated to DyLight649, anti-CD41 conjugated to PE and anti-CD62P conjugated to FITC. The movie shows the dynamic capture and release of platelets (red) by a crawling neutrophil (white), some of which are activated as indicated by the expression of CD62P (green).

Courtesy of Dr. Vinatha Sreeramkumar & Dr. Andrés Hidalgo (Centro Nacional de Investigaciones Cardiovasculares).

Spinning disk confocal with single- or dual- camera options and up to 6-position filter wheel for very high-speed 3D imaging (up to 10,000rpm/2,000fps).

Double-disk design with motorized bypass and large field of view, single- or dual-camera options and up to 10-position filter wheel for high speed 3D imaging.

CSU-W1 SoRa (Super-Resolution via Optical Re-Assignment) enables easy-to-use super-resolution imaging that “just works”.

Motorized spinning X,Y TIRF system for large-field, even TIRF illumination

Computer-generated holography system for photomanipulation via shapes, hand-drawn regions, and diffraction-limited spots in multiple 3D locations.

Diffraction limited high speed X,Y scanner for line and ROI interest scanning. Enables rapid direction of all photomanipulation including laser injury, uncaging, activation and bleaching.

Attenuatable 355nm or 532nm pulse laser (>60µJ pulses at 200Hz) capable of inflicting localized damage to a diffraction-limited spot in intracellular structures for precise laser ablation and laser wounding.

Modular laser light source with up to six (6) customizable solid state diode lasers and four (4) fiber optic outputs with sub-millisecond switching and on/off times.

Optical path selector with rapid 1ms selection of up to three (3) optical output (or input) paths for one input (or output) path.

Motorized, easy-to-use spherical aberration correction for mSwitcher.


TTL Synchronization (TTL Sync) provides sophisticated, reliable electronic control of carefully timed devices in a microscopy system with a combination of custom-designed TTL electronics and SlideBook software.

SlideBook digital microscopy software advances research microscopy through the entire experimental process. By managing everything from instrument control to image processing and data analysis, SlideBook allows scientists to focus on investigation rather than instrumentation.

Connect with us

Jan 2018
Berkeley City Club, Berkeley, CA
March 2018
Düsseldorf, Germany
March 2018
May 2018
Marine Biological Laboratory,
Woods Hole, MA

Let’s work together


Phone: +1 (303)-607-9429 x1

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Phone: +1 (303)-607-9429 x2

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Intelligent Imaging Innovations, Inc.

3509 Ringsby Ct

Denver, CO 80216, USA

Phone: +1 (303)-607-9429

Fax: +1 (303)-607-9430


Intelligent Imaging Innovations GmbH

Königsallee 9-21

D-37081 Göttingen, Germany

Phone: +49 (0)551-508-39-266

Fax: +49 (0)551-508-39-268

Intelligent Imaging Innovations Ltd

7 Westbourne Studios

242 Acklam Rd, London, UK W10 5JJ

Phone: +44 (0) 2037 440031