3i designs and manufactures technologies for living cell, live cell, and intravital fluorescence microscopy including superresolution, computer-generated holography, spinning disk confocal, multi-photon and lightsheet. SlideBook software manages everything from instrument control to image capture, processing and data analysis.
HeLa cells transfected with GFP-Lifeact (488nm) to visualize actin dynamics and stained with DiL (561nm red)(Thermo) to visualize membrane dynamics.
Microglia
Two- and three-photon microscopy of the corpus callosum in a 6-week old transgenic mouse which specifically labels oligodendrocytes (Mobp-EGFP).
Movie of neutrophil and platelets in the inflamed cremasteric venules of a WT mouse, 3h after local injection of TNFa. Cells are labeled by injection of low amounts of antibodies anti-Ly6G conjugated to DyLight649, anti-CD41 conjugated to PE and anti-CD62P conjugated to FITC. The movie shows the dynamic capture and release of platelets (red) by a crawling neutrophil (white), some of which are activated as indicated by the expression of CD62P (green).
Spinning disk confocal with single- or dual- camera options and up to 6-position filter wheel for very high-speed 3D imaging (up to 10,000rpm/2,000fps).
Double-disk design with motorized bypass and large field of view, single- or dual-camera options and up to 10-position filter wheel for high speed 3D imaging.
CSU-W1 SoRa (Super-Resolution via Optical Re-Assignment) enables easy-to-use super-resolution imaging that “just works”.
Computer-generated holography system for photomanipulation via shapes, hand-drawn regions, and diffraction-limited spots in multiple 3D locations.
Diffraction limited high speed X,Y scanner for line and ROI interest scanning. Enables rapid direction of all photomanipulation including laser injury, uncaging, activation and bleaching.
Attenuatable 355nm or 532nm pulse laser (>60µJ pulses at 200Hz) capable of inflicting localized damage to a diffraction-limited spot in intracellular structures for precise laser ablation and laser wounding.
Modular laser light source with up to six (6) customizable solid state diode lasers and four (4) fiber optic outputs with sub-millisecond switching and on/off times.
Optical path selector with rapid 1ms selection of up to three (3) optical output (or input) paths for one input (or output) path.
Intelligent Imaging Innovations, Inc.
3509 Ringsby Ct
Denver, CO 80216, USA
Phone: +1 (303)-607-9429
Fax: +1 (303)-607-9430
info@intelligent-imaging.comIntelligent Imaging Innovations GmbH
Königsallee 9-21
D-37081 Göttingen, Germany
Phone: +49 (0)551-508-39-266
Fax: +49 (0)551-508-39-268
3ieurope@intelligent-imaging.comIntelligent Imaging Innovations Ltd
7 Westbourne Studios
242 Acklam Rd, London, UK W10 5JJ
Phone: +44 (0) 2037 440031
3ieurope@intelligent-imaging.com