Advanced Multimodal Microscopy
Systems and Solutions

Established in 1995 by a group of cell biologists, neuroscientists, and computer scientists to provide advanced multi-dimensional microscopy systems for the biological research community, 3i designs and manufactures multimodal fluorescence microscopy systems for live cell, intravital, and cleared-specimen applications. 3i offers the latest methods and techniques ranging from super-resolution spinning disk confocal to spatial light modulator (SLM)-based holographic photomanipulation to multiphoton imaging to lightsheet. SlideBook software manages everything from instrument control to image capture, processing and data analysis in a single intuitive software package.

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Cleared Tissue LightSheet
High Speed High Resolution Imaging of Cleared Tissue and Whole Organs

Smooth muscle cells in the arteries from veins and capillaries of cleared mouse brain reported by NG2BacDsRed.

Sample courtesy of Dr. Woo-Ping Ge, University of Texas Southwestern Medical Center. Cleared with PEGASOS, imaged on Cleared Tissue LightSheet (CTLS), and rendered with Aivia.

Lattice LightSheet
Ultrathin light sheet for rapid
acquisition with minimal photodamage

HeLa cells transfected with GFP-Lifeact (488nm) to visualize actin dynamics and stained with DiL (561nm red)(Thermo) to visualize membrane dynamics.

Courtesy of James Springfield, Institute for Molecular Bioscience (IMB) at the University of Queensland.

Marianas LightSheet
Ultrathin light sheet for rapid acquisition with minimal photodamage


Courtesy of Dr. S. Okabe & Dr. S. Tanaka, Department of Cellular Neurobiology, University of Tokyo.

Inverted microscope platform for live cell research

Cell Division

Captured using 3i Marianas and CSU-W1 SoRa.

VIVO Multiphoton
Intravital Multiphoton Imaging System

Two- and three-photon microscopy of the corpus callosum in a 6-week old transgenic mouse which specifically labels oligodendrocytes (Mobp-EGFP).

Courtesy of Drs. Ethan Hughes, Emily Gibson, and Diego Restrepo at the University of Colorado Anschutz Medical Campus.


Neutrophils and platelets in the inflamed cremasteric venules of a WT mouse, 3h after local injection of TNFa. Cells are labeled by injection of low amounts of antibodies anti-Ly6G conjugated to DyLight649, anti-CD41 conjugated to PE and anti-CD62P conjugated to FITC. The movie shows the dynamic capture and release of platelets (red) by a crawling neutrophil (white), some of which are activated as indicated by the expression of CD62P (green).

Courtesy of Dr. Vinatha Sreeramkumar & Dr. Andrés Hidalgo (Centro Nacional de Investigaciones Cardiovasculares).

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Spinning disk confocal with single- or dual- camera options and up to 6-position filter wheel for very high-speed 3D imaging (up to 10,000rpm/2,000fps).


Double-disk design with motorized bypass and large field of view, single- or dual-camera options and up to 10-position filter wheel for high speed 3D imaging.

CSU-W1 SoRa (Super-Resolution via Optical Re-Assignment) enables easy-to-use super-resolution imaging that “just works”.


Motorized spinning X,Y TIRF system for large-field, even TIRF illumination


Computer-generated holography system for photomanipulation via shapes, hand-drawn regions, and diffraction-limited spots in multiple 3D locations.


Diffraction limited high speed X,Y scanner for line and ROI interest scanning. Enables rapid direction of all photomanipulation including laser injury, uncaging, activation and bleaching.

Attenuatable 355nm or 532nm pulse laser (>60µJ pulses at 200Hz) capable of inflicting localized damage to a diffraction-limited spot in intracellular structures for precise laser ablation and laser wounding.


Modular laser light source with up to six (6) customizable solid state diode lasers and four (4) fiber optic outputs with sub-millisecond switching and on/off times.

Optical path selector with rapid 1ms selection of up to three (3) optical output (or input) paths for one input (or output) path.

Motorized, easy-to-use spherical aberration correction for mSwitcher.


TTL Synchronization (TTL Sync) provides sophisticated, reliable electronic control of carefully timed devices in a microscopy system with a combination of custom-designed TTL electronics and SlideBook software.

SlideBook digital microscopy software advances research microscopy through the entire experimental process. By managing everything from instrumentcontrol to image processing and data analysis, SlideBook allows scientists to focus on investigation rather than instrumentation.

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    Phone: +1 (303)-607-9429 x1

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    Phone: +1 (303)-607-9429 x2

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    Intelligent Imaging Innovations, Inc.

    3575 Ringsby Ct, Suite 102

    Denver, CO 80216, USA

    Phone: +1 (303)-607-9429

    Fax: +1 (303)-607-9430


    Intelligent Imaging Innovations GmbH

    Königsallee 9-21

    D-37081 Göttingen, Germany

    Phone: +49 (0)551-508-39-266

    Fax: +49 (0)551-508-39-268

    Intelligent Imaging Innovations Ltd

    17 Westbourne Studios

    242 Acklam Rd, London, UK W10 5JJ

    Phone: +44 (0) 2037 440031

    Intelligent Imaging Innovations SAS

    17 rue Moreau

    75012 Paris, France

    Phone: +33 6 60 78 55 21