Customizable advanced
live-tissue imaging microscopy platform for neuroscience and cardiovascular studies

The VIVO platform incorporates advanced optics, cameras, computers and proprietary electronics to achieve unparalleled speed, precision and flexibility in live tissue image acquisition. The configuration of the VIVO system is designed to allow the presentation and maintenance of healthy live tissue without compromising high sensitivity imaging at high speeds. Innovative SlideBook software works seamlessly within VIVO for powerful and intuitive image acquisition and analysis.

Cardiovascular Studies:

Fast Imaging & Tissue Injury

VIVO is ideally suited for imaging vascular beds including dermis/skin-flap, mesentery, cremaster, cerebral-microvessels (via cranial window), and for imaging of larger vessels including the femoral and carotid arteries. The combination of high sensitivity and high rate of capture is essential for accurate measurement of dynamic cardiovascular events such as thrombus formation and leukocyte rolling.

VIVO comes standard with a stable, fixed, large working area stage compatible with mounting multiple manipulators and large trays for tissue and animal presentation. Optional Ablate! laser ablation system uses an attenuatable 355nm or 532nm pulse laser (>60µJ pulses at 200Hz) to enable controlled vessel damage and initiation of thrombus formation. Ablate! can be combined with Vector for directed motion including point & shoot.

In combination with spinning disk confocal (SDC), VIVO facilitates high speed optical sectioning and subsequent 3D reconstruction allowing for platelet and leukocyte applications.

Mouse cremaster arteriole with thrombus imaged with VIVO SDC.

Illustration of Ablate! laser-induced injury to blood vessel.

Neutrophils and platelets in the inflamed cremasteric venules of a WT mouse, 3h after local injection of TNFa. Cells are labeled by injection of low amounts of antibodies anti-Ly6G conjugated to DyLight649, anti-CD41 conjugated to PE and anti-CD62P conjugated to FITC. The movie shows the dynamic capture and release of platelets (red) by a crawling neutrophil (white), some of which are activated as indicated by the expression of CD62P (green).

Courtesy of Dr. Vinatha Sreeramkumar & Dr. Andrés Hidalgo (Centro Nacional de Investigaciones Cardiovasculares).

Stable. Fast. Flexible. Powerful.

VIVO System Animation

YOKOGAWA CSU-W1

CAMERAS

A variety of detector options include EMCCDs and high-speed high-sensitivity sCMOS cameras.

Motorized with customizable large under-objective workspace capable of adapting between thin tissue and slice preparation to live animals.

Large working area compatible with mounting multiple manipulators and large trays for tissue and animal presentation.

Laser ablation system with solid state pulsed laser for tissue damage, laser dissection and sub-cellular feature ablation. Optional rapid steering available when combined with Vector.

Platform-mounted spinning disk confocal with single- or dual-camera options and up to 10-position filter wheel for high speed 3D imaging.

RESEARCH UPRIGHT MICROSCOPE

PLATFORM STAGE

ABLATE!

Millisecond particle tracking

Key Components

LED Light Sources

Available high power 6-channel LED, 4-channel LED and white light LED sources for widefield illumination.

LaserStack

Compact modular solid state laser launch with

TTL Synchronization

Custom TTL electronics available
in rackmounted and compact USB form factors.

Vector

Diffraction limited high speed X,Y scanner for line and ROI interest scanning. Enables rapid direction of all photomanipulation including laser injury, uncaging, activation and bleaching.

Phasor

Computer-generated holography system for photomanipulation via shapes, hand-drawn regions, and diffraction-limited spots in multiple 3D locations.

Surgical Trays

Custom-designed surgical trays for presentation of exteriorised tissue and for stabilization of tissue presentation using optical windows such as a cranial window.

Yokogawa CSU-X1

Direct- or platform-mounted spinning disk confocal with single- or dual-camera options and up to 6-position filter wheel for very high speed 3D imaging (up to 10,000rpm/2,000fps).

Yokogawa CSU-W1

Platform-mounted spinning disk confocal with single- or dual-camera options and up to 10-position filter wheel for high speed 3D imaging.

Methods + Applications

The VIVO system is ideally suited for imaging vascular beds including: dermis/skin-flap, mesentery, cremaster, cerebral-microvessels (via cranial window) and for imaging of larger vessels including the femoral and carotid arteries. The combination of high-sensitivity and high rate of capture is essential for accurate measurement of dynamic cardiovascular events such as thrombus formation and leukocyte rolling.

VIVO comes standard with a stable, fixed, large working area stage compatible with mounting multiple manipulators and large trays for tissue and animal presentation. Optional Ablate! laser ablation system uses a pulsed 532nm laser (>60µJ pulses at 200Hz) to enable controlled vessel damage and initiation of thrombus formation. Ablate! is available in fixed-point and scanning versions.

In combination with Spinning Disk Confocal, VIVO facilitates high-speed optical sectioning and subsequent 3D reconstruction allowing for platelet and leukocyte applications.

Blastocyst

Captured with 3i VIVO system

Slidebook Software

SlideBook digital microscopy software advances research microscopy through the entire experimental process. By managing everything from instrument control to image processing and data analysis, SlideBook allows scientists to focus on investigation rather than instrumentation.

Key features for VIVO:

Point and shoot on-the-fly photoablation with Ablate! Double click with the mouse during live capture to trigger photoablation.

Particle tracking with rolling velocities and particle/cell numbers.

Streaming 4D capture with piezo objective collar eliminates delays between slices with continuous synchronized z-movement.

TTL synchronization module enables synchronized triggering and recording of external events directly in SlideBook metadata. For example, synchronize capture with breath or heartbeat to remove motion blur, or record temperature during imaging.

PTZ-induced seizures in the brain of Zebrafish larva

Calcium signals from the brain of Zebrafish larva in resting conditions (left) and after PTZ-induced seizures (right). The transgenic line is Tg(NeuroD:GCaMP6f). Image courtesy of Laura Desban, Andrew Prendergast and Claire Wyart. Captured with 3i VIVO and Yokogawa CSU-X1 SDC.

Image courtesy of Laura Desban, Andrew Prendergast and Claire Wyart. Captured with 3i VIVO and Yokogawa CSU-X1 SDC.

Specifications

MICROSCOPE

Research-grade fixed-stage upright microscope

CAMERA

Hamamatsu ORCA-Flash 4.0 v3 sCMOS
Photometrics Prime 95B sCMOS

SPINNING DISK CONFOCAL

Yokogawa CSU-X1 – up to 10,000rpm/2,000fps with available emission filter wheel and/or 2 camera ports
Yokogawa CSU-W1 – up to 4,000rpm/200fps with wide FOV, motorized bypass, and/or 2 camera ports

PHOTOABLATION

Ablate! 355nm or 532nm attenuable pulse laser (>60µJ at 200Hz)
Optional motorized steering

LED LIGHT SOURCES

Compatible with most LED light sources, including X-Cite TURBO LED 6-Channel Light Source

SOFTWARE

SlideBook software for acquisition and GPU-accelerated analysis

STAGE

Prior ZDeck system with ProScan XY stage (upgradeable with 50nm linear encoders)Sutter MPC-78 Platform Stage

LASER LINES

LaserStack – compact modular laser launch with up to 6 lasers from 405nm to 640nm

PHOTOSTIMULATION

Computer-generated holography: Phasor for simultaneous illumination of multiple arbitrary regions
Scanning: Vector high-speed XY scanner for point or spiral stimulation

SPECIMEN PREPARATION

Custom tissue preparation and mounting trays for easy and stable mounting of the tissue of interest.
A glass optical window allows high quality brightfield including DIC.

ENVIRONMENTAL CONTROL

Available perfusion system and full chamber incubator

Mouse heart imaged with VIVO SDC. Green = FITC-BSA. Red = Gr-1+ Neutrophils.

Image courtesy Dr. Dean Kavanagh, University of Birmingham.

Mouse lung imaged with VIVO and widefield illumination. Green = leukocytes.

Image courtesy of Dr. Christophe Dubois, Faculté de Pharmacie de Marseille.

Mouse kidney imaged with VIVO SDC.

Learn more
about VIVO

SALES

Phone: +1 (303)-607-9429 x1
Email: sales@intelligent-imaging.com

Profile on Ariba Discovery

SUPPORT