Lattice LightSheet

A Microscope for High-Resolution, Fast, and Gentle 3D Live Cell Imaging

Break the Diffraction Limit with SIM

Single-Molecule Imaging

Photomanipulation

Highlights

Super-Resolution | Break the Diffraction Limit with SIM

Dithered Sheet Scan

The lightsheet is rapidly dithered along the X axis and one image is captured per Z plane.

Structured Illumination Microscopy (SIM):

The lightsheet is stepped along 5 discrete phase steps along the X axis. Five raw images are collected that are reconstructed to produce an image that is beyond the diffraction limit of the detection objective.

Both dithered and SIM capture can be performed via a sample scan, where the sample is stepped in Z, or in a galvo/objective scan, where the lightsheet and objective are stepped through the sample.

 
Super-Resolution
Critical nuclear processes are more easily visualized by pushing past the diffraction limit of resolution on the Lattice LightSheet. Here, condensed and uncondensed DNA is visualized via SIM for a clearer picture of DNA packaging around histones. Time-lapse SIM imaging of DNA in mouse embryonic stem cell stained with JF646–Hoechst, courtesy of Liang-Fu Chen and Joanna Wysocka at Stanford

Photomanipulation

Photomanipulation is a powerful tool for studying membrane dynamics, receptor and vesicle movement, and photo conversion of specific molecules. The integration of Vector2 X,Y galvo scanner onto the Lattice LightSheet expands the capabilities of an already impressive system. As a market leader in photomanipulation, 3i makes complicated bleaching, FRAP, uncaging, and photoconversion experiments easy. Vector2 slots seamlessly into SlideBook for complete control on new or existing Lattice LightSheet systems.

 
photomanipulation
Bleaching of a cell membrane via Vector2. Targeting of specific regions of interest is highlighted by the flashes of excitation light in a line across the sample, repeated over a few time points to gently bleach out the target portion of the sample. Bleaching is possible with any laser present on the system.

 
frap
Fluorescence Recovery After Photobleaching (FRAP) can be used to study kinetics of membrane diffusion after a targeted region has been bleached of fluorescent signal. Over time the fluorescence in the targeted area recovers based on the rate of diffusion.

Single-Molecule Imaging

Cell biologists and biophysicists are increasingly interested in tracking single molecules of interest instead of looking at groups of proteins as a unit. Single molecule localization microscopy (SMLM) offers researchers the ability to observe individual fluorophores on a single molecule of interest and observe how each behaves. Typically, techniques like TIRF have been required for SMLM but Lattice LightSheet offers the following capabilities to increase overall signal-to-noise and facilitate SMLM imaging in 3D:

  • High-powered lasers (up to 2W)
  • The most sensitive sCMOS cameras with low read noise and 95% QE
  • 100+ fps acquisition for rapid single plane imaging or acquiring volumes per second
  • Photoswitching and photoconversion through Vector2

Intracellular to Extracellular to Multicellular Imaging

With 17 different light sheet lengths and thicknesses to pick from, it is easy to find the ideal Lattice for your sample.

Light sheet lengths from 15-75 µm

  • 15-20 µm sheets for intracellular events
  • 30-40 µm sheets for small organoids and cell-cell interactions
  • 50-75 µm sheets for large organoids, portions of embryos, and parts of larger organisms

Using the “Mask” mode allows for changing of the lightsheet while your sample remains in focus, allowing users to evaluate different light sheet parameters in real time.

SIM is possible with any of the sheets, opening the door for fast super-resolution imaging of an assortment of sample types.

 
Mitochondria
Mitochondrial fusion and fission is a rapid cellular process that is also indicative of cellular damage from phototoxicity. The gentle nature of the Lattice LightSheet slows for unprecedented, volumetric imaging of this dynamic process without photodamage. Mitotracker Red in RPE1 cell.

 
Immunology
Visualizing cell-cell interactions is commonly displayed using a volume rendering. Here a slice/ortho view across X, Y, and Z allows for detailed examination of each cell’s membrane due to the high axial resolution offered by the Lattice LightSheet. Jurkat cell (red) interacting with HeLa cell (green).

 
MULTICELLULAR
Medium-sized samples are easy to accommodate via longer light sheets, all controlled digitally. Drosophila primary neurons with Jupiter (orange) and CD8 (blue).

 
Plant Biology
Large samples, even with light scattering cell walls, can still be visualized with the Lattice LightSheet. Taking advantage of the longer 75 µm long sheet, a portion of a root tip expressing GFP tubulin is imaged in high resolution.

Technology

First developed by Nobel Laureate Dr. Eric Betzig, the 3i Lattice LightSheet microscope is capable of imaging biological systems spanning four orders of magnitude in space and time. The system generates an optical lattice to create an ultra-thin light sheet to image biological samples over long periods of time and with very fine resolution. This allows for 4D living cell imaging, where experiments limited to seconds or minutes on other imaging platforms can be extended to hours or even days. The combination of high spatiotemporal resolution, imaging speed and sensitivity make Lattice LightSheet the ultimate imaging tool for a new era of living cell microscopy.

Beam Structuring and Shaping via Spatial Light Modulator and Annular Mask

As described in Chen, Science 2014, the Lattice LightSheet is a system designed to push the spatial and temporal resolution limits in live cell imaging. 3i has improved upon the original design from Dr. Eric Betzig with the goal of making the system more user friendly.

 

1. Cylindrical lenses stretch and collimate the beam to form a sheet projected onto a spatial light modulator (SLM).
2. SLM generates an optical lattice of Bessel beams.
3. Annular mask acts as a zero order filter, removing artifacts and lengthening the sheet.
4. Galvos dither the sheet in X and sweep in Z.
5. Emitted fluorescence is detected on a high QE, low read-noise, sCMOS camera

 

Optimal Lightsheet Illumination

Because the light is entering the specimen along the plane of focus, the likelihood of a useful fluorescence event is far higher than in other methods that illuminate through the cell.

Custom-made LWD illumination objective

Custom-made 0.71NA long working distance water immersion objective for lightsheet illumination, mechanically and optically matched to the imaging objective.

High resolution 1.1NA imaging objective

High-resolution 1.1NA water immersion objective with depth of field matched to lightsheet thickness for excellent optical sectioning.

 0.4µm lightsheet thickness      0.5µm objective depth of field

A Lattice lightsheet formed in fluorescent dye, viewed from the imaging objective.

Making an Optical Lattice

Gaussian

Bessel

Lattice

A lightsheet as thin as 400 nm with minimal out-of-focus excitation.

Uniformly thin along its length.

Can be used for super-resolution through structured illumination microscopy (SIM).

 
Interference patterns of differently spaced Bessel beams. With an ideal spacing/periodicity for each wavelength and annular mask position, a Lattice pattern can be formed.

Features for Advanced Lightsheet Imaging

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Motorized Sample Chamber

Objectives remain fixed and aligned

Rapid and repeatable sample changing

Expanded specimen access when loading

Load and home positions for 2-move sample change

Removable inner chamber for easy cleaning

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SOLID STATE HEATING SYSTEM

Accurate and stable temperature setting for the objectives and sample holder

No water, tubing, heaters or pumps

Active feedback for improved thermal stability

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OKOLAB INCUBATOR UPGRADE

Complete Okolab Incubator with Bold Line temperature controller, heater and digital CO2 gas mixer for comprehensive environmental control

Includes active humidity controller (50-95%) and heated sample chamber and objective assembly with easy to use Oko-Touch controller.

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LED BRIGHTFIELD &
EPI-FLUORESCENCE

Proper epi-fluorescence illumination via LEDs, mirrors and filters behind the vertical board

Significant reduction in light dose compared to laser epi-excitation

Multi-band dichroic replaces 90/10 beamsplitter

Multi-channel LED illuminator to match the imaging lasers

White brightfield LED illumination through excitation objective

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UP TO 6 LASERS

SlideBook Software for Lattice LightSheet Imaging

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UP TO 6 LASERS

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Can be customized to your preferred color scheme

Aivia Advanced Rendering and Analysis

SlideBook offers a solid set of tools for 3D rendering and analysis. For advanced rendering and analysis 3i offers Aivia. Aivia is an innovative and complete 2D-to-5D image visualization, analysis and interpretation platform. Using state-of-the-art algorithm and software architecture, Aivia delivers top performance on critical tasks such as display of large images and analysis of complex biological phenomena. Aivia is powered by a range of machine learning technology for both image segmentation, object classification and novelty detection.

Powerful Computer Workstation

3i provides high-power computer workstations to control all microscope hardware necessary for acquisition as well as enable processing, segmentation and volume rendering of terabyte (TB) datasets without additional computer resources.  Solid state drives in RAID configurations provide high-speed storage for capture, while traditional high-capacity hard drives provide longer-term storage of datasets. NVIDIA Quadro GPUs work in parallel with the latest Intel Xeon processors for fast stitching, processing and rendering of captured data.

Petabyte Data Storage

3i offers DDN® unified storage systems to allow direct acquisition and analysis, without time-consuming file transfers, at volumes ranging from 500TB to over 2PB. DDN storage systems are an ideal choice for labs and facilities looking to optimize acquisition workflows and/or incorporate data analysis pipelines.

Lattice LightSheet Data Sets

 
Mitosis
The near isotropic resolution of the Lattice LightSheet allows for incredible videos of mitotic cells in 4 dimensions. Mammalian cells expressing H2B-RFP. Courtesy of Dr. Andrew McAnish, University of Warwick.
 
Membrane Dynamics
Actin ruffles on a large macrophage imaged at 1 volume/second to capture rapid membrane dynamics over a large cell. Macrophage with LifeAct GFP. Courtesy of Dr. James Springfield, University of Queensland.
 
Neuroscience
Imaging the process of neuronal axon lengthening requires a gentle excitation source and rapid volumetric imaging. Sample was imaged at >1 volume/second to capture rapid microtubule dynamics in the growth cone of a primary rat neuron.
 
Hematology
Platelet generation is typically a difficult process to capture using fluorescent microscopy – the megakaryocytes won’t produce platelets if they are excited with too much laser light. Here, the Lattice LightSheet is gentle enough to image this unique developmental process at high resolution. Courtesy of Dr. Watson, University of Birmingham.
 
Model Organism
Dictyostelium are a “slime mold” that tend to run away from exciation light before they begin to photobleach. The Lattice LightSheet is gentle and fast enough to image dictyostelium at over a volume/second without the cells migrating away. This allows for unprecedented imaging of this model organism. Dictyostelium expressing LifeActGFP. Courtesy of Dr. Till Bretschneider, University of Warwick.
 
cytoskeleton
Microtubules are the ever changing cytoskeleton present in the cell. Here, fast volumetric imaging of the microtubule network displays distinct differences in the cytoskeletal dynamics through out the cell. MCF-7 cells transfected with GFP-EMTB to visualize microtubule dynamics. Courtesy of Dr. Gordon L. Hager and David A. Garcia at the National Cancer Institute.

Specifications

LIGHT SHEET THICKNESS

0.4µm at 50µm length

DETECTION OPTICS

1.1NA water objective, 2.0mm WD, 62.5x total magnification

ILLUMINATION OPTICS

0.71NA water objective, 3.7mm WD

LASER OPTIONS

Up to 6 laser lines, wavelengths from 405 – 670 nm, powers from 100 – 2000 mW (depending on wavelength)

STANDARD CAMERA

Hamamatsu ORCA-Fusion BT sCMOS Camera

CAMERA OPTIONS

Single sCMOS, Dual sCMOS direct 1x projection, Dual EMCCD relayed 2.5x projection

SAMPLE CHAMBER

Medical grade stainless steel with TEC temperature control and perfusion capabilities. Optional – complete environmental control system with active temperature, humidity, and CO2 control via Okolab.

SPECIMEN MOUNTING

Standard, horizontally-oriented 5mm round coverslip

ACQUISITION COMPUTER

Dual 16-Core Xeon Gold 2.9GHz processors, 128GB RAM, 8GB NVIDIA Quadro RTX4000 workstation graphics card, 512GB OS SSD, 8TB Fast Acquisition Drive, and 20TB additional storage

ANALYSIS COMPUTER

Dual 26-Core Xeon Gold 2.1GHz processors, 256GB RAM, 24GB NVIDIA Quadro RTX6000 workstation graphics card, 512GB OS SSD, 8TB Fast Acquisition Drive, and 20TB additional storage

STORAGE SOLUTIONS

DDN® unified storage systems for direct full-speed acquisition and analysis starting at 500TB. DDN systems utilize a BioScaler GPFS file system and are easily expandable to multiple petabytes

Publication Spotlight

CENP-F Stabilizes Kinetochore-Microtubule Attachments and Limits Dynein Stripping of Corona Cargoes.

Auckland, Philip, Emanuele Roscioli, Helena Louise Elvidge Coker, and Andrew D. McAinsh. “CENP-F Stabilizes Kinetochore-Microtubule Attachments and Limits Dynein Stripping of Corona Cargoes.” Journal of Cell Biology 219, no. 5 (May 4, 2020).

https://doi.org/10.1083/jcb.201905018

Insight from the Maximal Activation of the Signal Transduction Excitable Network in Dictyostelium Discoideum.

Edwards, Marc, Huaqing Cai, Bedri Abubaker-Sharif, Yu Long, Thomas J. Lampert, and Peter N. Devreotes. “Insight from the Maximal Activation of the Signal Transduction Excitable Network in Dictyostelium Discoideum.” Proceedings of the National Academy of Sciences of the United States of America 115, no. 16 (17 2018): E3722–30.

https://doi.org/10.1073/pnas.1710480115

Oxygen Tension and the VHL-Hif1α Pathway Determine Onset of Neuronal Polarization and Cerebellar Germinal Zone Exit.

Kullmann, Jan A., Niraj Trivedi, Danielle Howell, Christophe Laumonnerie, Vien Nguyen, Shalini S. Banerjee, Daniel R. Stabley, Abbas Shirinifard, David H. Rowitch, and David J. Solecki. “Oxygen Tension and the VHL-Hif1α Pathway Determine Onset of Neuronal Polarization and Cerebellar Germinal Zone Exit.” Neuron, March 3, 2020.

https://doi.org/10.1016/j.neuron.2020.02.025

Ordered and Disordered Segments of Amyloid-β Drive Sequential Steps of the Toxic Pathway.

Maity, Barun Kumar, Anand Kant Das, Simli Dey, Ullhas Kaarthi Moorthi, Amandeep Kaur, Arpan Dey, Dayana Surendran, et al. “Ordered and Disordered Segments of Amyloid-β Drive Sequential Steps of the Toxic Pathway.” ACS Chemical Neuroscience 10, no. 5 (15 2019): 2498–2509.

https://doi.org/10.1021/acschemneuro.9b00015

Lattice Light-Sheet Microscopy Multi-Dimensional Analyses (LaMDA) of T-Cell Receptor Dynamics Predict T-Cell Signaling States.

Rosenberg, Jillian, Guoshuai Cao, Fernanda Borja-Prieto, and Jun Huang. “Lattice Light-Sheet Microscopy Multi-Dimensional Analyses (LaMDA) of T-Cell Receptor Dynamics Predict T-Cell Signaling States.” Cell Systems 10, no. 5 (May 20, 2020): 433-444.e5.

https://doi.org/10.1016/j.cels.2020.04.006

Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14.

Surcel, Alexandra, Eric S. Schiffhauer, Dustin G. Thomas, Qingfeng Zhu, Kathleen T. DiNapoli, Maik Herbig, Oliver Otto, et al. “Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14.” Cancer Research 79, no. 18 (September 15, 2019): 4665–78.

https://doi.org/10.1158/0008-5472.CAN-18-3131

Rab8a Localisation and Activation by Toll-like Receptors on Macrophage Macropinosomes.

Wall, Adam A., Nicholas D. Condon, Lin Luo, and Jennifer L. Stow. “Rab8a Localisation and Activation by Toll-like Receptors on Macrophage Macropinosomes.” Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 374, no. 1765 (04 2019): 20180151.

https://doi.org/10.1098/rstb.2018.0151

Macropinosome Formation by Tent Pole Ruffling in Macrophages.

Condon, Nicholas D., John M. Heddleston, Teng-Leong Chew, Lin Luo, Peter S. McPherson, Maria S. Ioannou, Louis Hodgson, Jennifer L. Stow, and Adam A. Wall. “Macropinosome Formation by Tent Pole Ruffling in Macrophages.” The Journal of Cell Biology 217, no. 11 (05 2018): 3873–85.

https://doi.org/10.1083/jcb.201804137

Chapter Five – Imaging Intercellular Interaction and Extracellular Vesicle Exchange in a Co-Culture Model of Chronic Lymphocytic Leukemia and Stromal Cells by Lattice Light-Sheet Fluorescence Microscopy.

Elgamal, Sara, Federico Colombo, Francesca Cottini, John C. Byrd, and Emanuele Cocucci. “Chapter Five – Imaging Intercellular Interaction and Extracellular Vesicle Exchange in a Co-Culture Model of Chronic Lymphocytic Leukemia and Stromal Cells by Lattice Light-Sheet Fluorescence Microscopy.” In Methods in Enzymology, edited by Sheila Spada and Lorenzo Galluzzi, 645:79–107. Extracellular Vesicles. Academic Press, 2020.

https://doi.org/10.1016/bs.mie.2020.06.015

Mutually Inhibitory Ras-PI(3,4)P2 Feedback Loops Mediate Cell Migration.

Li, Xiaoguang, Marc Edwards, Kristen F. Swaney, Nilmani Singh, Sayak Bhattacharya, Jane Borleis, Yu Long, Pablo A. Iglesias, Jie Chen, and Peter N. Devreotes. “Mutually Inhibitory Ras-PI(3,4)P2 Feedback Loops Mediate Cell Migration.” Proceedings of the National Academy of Sciences of the United States of America 115, no. 39 (25 2018): E9125–34.

https://doi.org/10.1073/pnas.1809039115

Frontline Science: Dynamic Cellular and Subcellular Features of Migrating Leukocytes Revealed by in Vivo Lattice Lightsheet Microscopy.

Manley, Harriet R., David L. Potter, John M. Heddleston, Teng-Leong Chew, M. Cristina Keightley, and Graham J. Lieschke. “Frontline Science: Dynamic Cellular and Subcellular Features of Migrating Leukocytes Revealed by in Vivo Lattice Lightsheet Microscopy.” Journal of Leukocyte Biology, April 23, 2020.

https://doi.org/10.1002/JLB.3HI0120-589R

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy.

Rosenberg, Jillian, and Jun Huang. “Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy.” Journal of Visualized Experiments: JoVE, no. 155 (January 30, 2020).

https://doi.org/10.3791/59914

Drebrin-Mediated Microtubule-Actomyosin Coupling Steers Cerebellar Granule Neuron Nucleokinesis and Migration Pathway Selection.

Trivedi, Niraj, Daniel R. Stabley, Blake Cain, Danielle Howell, Christophe Laumonnerie, Joseph S. Ramahi, Jamshid Temirov, Ryan A. Kerekes, Phillip R. Gordon-Weeks, and David J. Solecki. “Drebrin-Mediated Microtubule-Actomyosin Coupling Steers Cerebellar Granule Neuron Nucleokinesis and Migration Pathway Selection.” Nature Communications 8 (23 2017): 14484.

https://doi.org/10.1038/ncomms14484

Integrating Chemical and Mechanical Signals through Dynamic Coupling between Cellular Protrusions and Pulsed ERK Activation.

Yang, Jr-Ming, Sayak Bhattacharya, Hoku West-Foyle, Chien-Fu Hung, T.-C. Wu, Pablo A. Iglesias, and Chuan-Hsiang Huang. “Integrating Chemical and Mechanical Signals through Dynamic Coupling between Cellular Protrusions and Pulsed ERK Activation.” Nature Communications 9, no. 1 (07 2018): 4673.

https://doi.org/10.1038/s41467-018-07150-9

Additional Resources

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    Email: sales@intelligent-imaging.com

    Phone: +1 (303)-607-9429 x1

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