The VectorTIRF hardware allows for TIRF imaging with high speed and unrivaled clarity on a large, evenly illuminated field of view via spinning the excitation light around the back aperture. VectorTIRF can be used for a variety of live-cell, in vitro, and even single molecule imaging when combined with a modern sCMOS or EMCCD detector. The flexibility of VectorTIRF allows integration onto numerous inverted microscope frames and is easily combined with other 3i hardware, creating the possibility for a single, powerful, spinning disk confocal, TIRF, FRAP, and ablation system. SlideBook software allows for easy sample finding, acquisition, and analysis.
Fixed position TIRF slider
Supported biphastic lipid layer (6nm thick) with fixed-position TIRF slider illumination.
Motorized Spinning X, Y TIRF Illumination, motorized spinning TIRF illumination with 5ms rotation speed and 20ms exposure.
405nm, 445nm, 488nm, 515nm, 561nm, 594nm, 640nm
>20 mW at the back aperture
Mounts to the sideport on Zeiss, Olympus, Nikon and Leica microscopes, including hardware autofocus
SlideBook software for acquisition and analysis
Polarization input adapter for imaging with polarized TIRF