Solutions for Optogenetics

Phasor Computer Generated Holography

Phasor is the only commercially available system capable of simultaneously illuminating multiple arbitrary regions in 3D with the use of SLM-based computer-generated holography (CGH).

Phasor adds precise and powerful photostimulation with advanced optical correction capabilities for maximum control of region shape, intensity, and location in 3D.

Phasor with LaserStack enables reliable, rapid 1P stimulation with up to 7 wavelengths. Visible wavelengths can be combined with near-IR for 2P uncaging at 720 nm.

Phasor 2P provides near-IR stimulation for deeper penetration in scattering tissue and 2-photon optical sectioning limits out-of-focus excitation. Zero-order management and diffraction correction produce an even power distribution with no dark spot.

Temporal Focusing for 2P confines the axial illumination to a thickness less than 20µm, resulting in a consistent z resolution independent of region size.

Phasor gets the most out of cutting-edge optogenetics tools

Phasor CGH Scanned SLM Scanning
Features
Single Spot
Spiral Stimulation
Simultaneous multiple Z locations
Simultaneous multi-spot stimulation
Simultaneous multi-region stimulation with arbitrary regions
Hand-draw arbitrary patterns in software
Upload pattern image in software
Uniform intensity distribution across field
Different intensity for different regions
Optical aberration corrections
Multiphoton zero-order management (no dark spot)
Imaging and photostimulation at different planes
Available temporal focusing upgrade

VIVO Multiphoton with

Vector RS+

All-optical multiphoton imaging system with integrated photostimulation

Vector RS+ hybrid scanner combines the speed of resonant scanning with the flexibility of dual galvanometers.

Vector RS+ allows rapid switching between 30 fps full-frame resonant scanning and photostimulation.

A dedicated input accommodates visible or near-IR wavelengths.

Vector Laser-Based ROI Scanner

Standalone Vector scanner can be added to a confocal or widefield system for spot and spiral photostimulation.

In addition, Vector easily incorporates visible wavelengths into a dual galvanometer VIVO Multiphoton system.

SlideBook Software

SlideBook seamlessly combines hardware control, photostimulation and image acquisition, allowing for quick, easy and repeatable experimental setup.

 

Easy-to-use Phasor module allows users to quickly and simply define regions directly in SlideBook and includes a proprietary iterative Fourier transform algorithm for generating holograms.

 

Built-in analysis tools can be combined with native scripting or bi-directional MATLAB integration.

SlideBook seamlessly combines hardware control, photostimulation and image acquisition, allowing for quick, easy and repeatable experimental setup.

 

Easy-to-use Phasor module allows users to quickly and simply define regions directly in SlideBook and includes a proprietary iterative Fourier transform algorithm for generating holograms.

 

Built-in analysis tools can be combined with native scripting or bi-directional MATLAB integration.

Let’s work together.






SALES

Phone: +1 (303)-607-9429 x1
Email: sales@intelligent-imaging.com

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SUPPORT

Phone: +1 (303)-607-9429 x2
Email: support@intelligent-imaging.com

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NORTH AMERICA

Intelligent Imaging Innovations, Inc.
3509 Ringsby Ct
Denver, CO 80216, USA
Phone: +1 (303)-607-9429
Fax: +1 (303)-607-9430
Email: info@intelligent-imaging.com

EUROPE

Intelligent Imaging Innovations GmbH
Königsallee 9-21
D-37081 Göttingen, Germany
Phone: +49 (0)551-508-39-266
Fax: +49 (0)551-508-39-268
3ieurope@intelligent-imaging.com

 

Intelligent Imaging Innovations Ltd
20-22 Wenlock Road
London, UK
Phone: +44 (0) 2037 440031
3ieurope@intelligent-imaging.com