Diffraction limited resolution in 3 axes with molecular group sensitivity and unprecedented light sheet specimen accessibility

Marianas LightSheet™ merges the low phototoxicity and large specimen handling of dual inverted selective plane illumination (diSPIM) with the power and flexibility of a live-cell microscope system.


diSPIM technology enables rapid 3D imaging of samples ranging from single cells to small organisms over the course of hours to days. Unlike capillary-based light sheet methods, diSPIM allows for standard specimen preparation in standard dishes in standard media.


Combined with spinning disk confocal, TIRF, FLIM and photomanipulation, Marianas LightSheet is a powerful live-cell imaging workstation. SlideBook™ hardware and software integration seamlessly manages acquisition, alignment, deconvolution and rendering.

HeLa cells with fluorescently labeled Actin and DNA.

Rapid 3D imaging
with isotropic resolution.

Selective plane illumination (SPIM) uses a thin sheet of light to illuminate only the plane of interest, reducing phototoxicity by drastically cutting total light dose and allowing for prolonged specimen imaging.


diSPIM employs two orthogonal objectives positioned at 45° above the specimen plane. By alternating between the objectives for imaging and excitation, diSPIM captures two volumes that are fused and deconvolved to achieve isotropic resolution.


The sheet is swept through the sample at high speed while a piezo moves the imaging objective to capture data up to 600 images per second.

The most flexible
sample accommodation
of any light sheet.

Horizontal specimen mounting supports conventional preparations such as coverslips and Petri dishes and provides a platform for custom-designed chambers.


Open sample chamber allows specimens of various sizes, ranging from cells to whole organisms.


Inverted microscope enables long term live cell experiments with available perfusion and incubator for full environmental control.

Drosophila embryo with DAPI staining of DNA.

The ideal solution for cell viability.


Peak light intensity is greatest in point-to-point scanning methods. Total light dose increases in methods that illuminate the entirety of the cell.


SPIM is ideal for long-term live cell imaging due to the low light intensity
compared to confocal and laser scanning systems.


Fully integrated software solution

Capture, fuse, deconvolve, analyze.

Integrated hardware and software control creates a seamless user experience with comprehensive control of acquisition and rendering.


•  Software assisted camera and laser alignment


•  High-speed deconvolution, fusion and 3D reconstruction


•  Custom scripting and MATLAB® integration

4 day old live zebra fish larva expressing GFP



10X water immersion, 0.3 NA, 3.5mm WD

20X water immersion, 0.5 NA, 3.5mm WD

40X water immersion, 0.8 NA, 3.5mm WD

16X multi-immersion RI 1.33-1.56, 0.4 NA, 12mm WD, for Cleared Specimens



150µm or 300µm piezo translators



Horizontal stainless steel chamber

50mm x 24mm coverglass standard

95mm Petri dish

Custom chambers available upon request


2048×2048 16-bit sCMOS 100fps

Liquid cooling



405nm, 445nm, 488nm, 515nm, 561nm, 594nm, 640nm



Available perfusion system and full chamber incubator



Dual 12-Core Xeon 2.3GHz CPUs

128GB RAM, 4GB GPU, 512GB solid state drive

2TB SSD RAID-10 drive array for data acquisition

10Gb Ethernet with optional multi-mode and single-mode fiberoptic connectivity

Find out more about
Marianas LightSheet


Phone: +1 (303)-607-9429 x1
Email: sales@intelligent-imaging.com

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Phone: +1 (303)-607-9429 x2
Email: support@intelligent-imaging.com

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