Marianas LightSheet™ merges the low phototoxicity and large specimen handling of dual inverted selective plane illumination (diSPIM) with the power and flexibility of a live-cell microscope system.
diSPIM technology enables rapid 3D imaging of samples ranging from single cells to small organisms over the course of hours to days. Unlike capillary-based light sheet methods, diSPIM allows for standard specimen preparation in standard dishes in standard media.
Combined with spinning disk confocal, TIRF, FLIM and photomanipulation, Marianas LightSheet is a powerful live-cell imaging workstation. SlideBook™ hardware and software integration seamlessly manages acquisition, alignment, deconvolution and rendering.
Selective plane illumination (SPIM) uses a thin sheet of light to illuminate only the plane of interest, reducing phototoxicity by drastically cutting total light dose and allowing for prolonged specimen imaging.
diSPIM employs two orthogonal objectives positioned at 45° above the specimen plane. By alternating between the objectives for imaging and excitation, diSPIM captures two volumes that are fused and deconvolved to achieve isotropic resolution.
The sheet is swept through the sample at high speed while a piezo moves the imaging objective to capture data up to 600 images per second.
Horizontal specimen mounting supports conventional preparations such as coverslips and Petri dishes and provides a platform for custom-designed chambers.
Open sample chamber allows specimens of various sizes, ranging from cells to whole organisms.
Inverted microscope enables long term live cell experiments with available perfusion and incubator for full environmental control.
Peak light intensity is greatest in point-to-point scanning methods. Total light dose increases in methods that illuminate the entirety of the cell.
SPIM is ideal for long-term live cell imaging due to the low light intensity
compared to confocal and laser scanning systems.
Integrated hardware and software control creates a seamless user experience with comprehensive control of acquisition and rendering.
• Software assisted camera and laser alignment
• High-speed deconvolution, fusion and 3D reconstruction
• Custom scripting and MATLAB® integration
10X water immersion, 0.3 NA, 3.5mm WD
20X water immersion, 0.5 NA, 3.5mm WD
40X water immersion, 0.8 NA, 3.5mm WD
16X multi-immersion RI 1.33-1.56, 0.4 NA, 12mm WD, for Cleared Specimens
150µm or 300µm piezo translators
Horizontal stainless steel chamber
50mm x 24mm coverglass standard
95mm Petri dish
Custom chambers available upon request
2048×2048 16-bit sCMOS 100fps
405nm, 445nm, 488nm, 515nm, 561nm, 594nm, 640nm
Available perfusion system and full chamber incubator
Dual 12-Core Xeon 2.3GHz CPUs
128GB RAM, 4GB GPU, 512GB solid state drive
2TB SSD RAID-10 drive array for data acquisition
10Gb Ethernet with optional multi-mode and single-mode fiberoptic connectivity