Marianas incorporates advanced optics, cameras, computers and proprietary electronics to achieve unparalleled speed, precision and flexibility in live cell image acquisition. Innovative SlideBook software works seamlessly within Marianas for powerful and intuitive image acquisition and analysis. An ever-growing lineup of 3i technologies and our team of experienced scientists and programmers ensure that Marianas is always a step above the rest.
The Marianas platform is equipped for both live and fixed cell widefield epifluorescence applications as well as transmitted illumination techniques.
Common uses include:
• Timelapse
• 3D deconvolution
• Large format montage
• Ratiometric imaging
• Multidimensional imaging (6D applications)
• FRET
• Stereology
MDCK cell line stained for DNA, alpha-tubulin and E-cadherin
Pellman lab, Dana-Farber Cancer Institute and Harvard Medical School
Advanced options
Advanced options further expand the capabilities of this system to address a wide variety of applications.
• Spinning Disk Confocal
• FRAP
• Rapid 4D
• Photoactivation and uncaging
• TIRF
• FLIM
• Single molecule studies
• Real-time deconvolution
• Environmental control
• Simultaneous dual camera acquisition and synchronization with electrophysiology and automated perfusion systems.
Pancreatic Islet Cell
Customize your system
CORE COMPONENTS
Research Inverted
Microscope
Fully motorized and capable
of autofocus and all
advanced techniques, with
a full suite of world class
objectives and filters.
LED Light Source
High power 6-channel
LED light source.
XYZ Stage
Fully motorized
multi-specimen platform
with options for linear
encoder sensitivity and
piezo z focus.
Computer
Dell professional grade
workstations offer
best-in-class speed and
large dataset handling.
Cameras
sCMOS, EMCCD and
intensified EMCCD options
for high resolution,
low light sensitivity and
frequency domain FLIM.
SlideBook
SlideBook offers advanced
acquisition via control of
hundreds of devices, in addition
to advanced analytical
techniques and 3D rendering.
ADVANCED TECHNOLOGIES
Spinning Disk Confocal
Yokogawa high speed CSU-X1
and wide field-of-view
CSU-W1 offer sensitive and
flexible 3D imaging solutions
for live cell microscopy.
LaserStack + FiberSwitcher
Modular solid state laser launch
with <1ms changing between
SDC, TIRF and Photomanipulation.
Frequency Domain FLIM
Modulated lasers and
ICCD camera allow for
video rate FLIM with high
spatial resolution.
TTL Synchronization
Custom TTL electronics
available in rack-mounted and
compact USB form factors.
VectorTIRF
VectorTIRF provides motorized
X,Y TIRF illumination
Incubator
Full enclosure with
temperature controller, heater
and CO2 gas mixer.
MULTI-MODAL CAPABILITIES
mSwitcher
Millisecond fast optical
path selection between
3 devices and microscope.
mSAC
High speed spherical
aberration correction as
3D data is collected.
Bifurcator
Spectral separation
or combination in a
perfect image relay.
Optimizer
Optimal positioning of
emission filter wheels in a
collimated infinity space.
PHOTOMANIPULATION
Vector
Diffraction limited high speed
X,Y scanner for line and
ROI interest scanning of
laser-based photomanipulation
light sources.
Phasor
Digital holography pattern
photomanipulation in X,Y and Z
without intensity loss.
Ablate
Laser ablation system with
532nm pulsed laser.