Custom built solutions

Our modular systems are designed
to enable custom configurations to adapt
to your research needs.

LaserStack

Modular Laser Light Source

mSwitcher

Optical Path Selector

mSAC

Spherical Aberration Correction

TTL Sync

TTL Synchronization

VectorTIRF

Motorized Spinning X,Y TIRF Illumination

Bifurcator

Optical Splitter and Combiner

Optimizer

Perfect Image Relay

DeMuxer

Spectral Separation

LaserStack

LaserStack is a laser launch system combining the output from a selection of ten solid state diode lasers and coupling them to a microscope via fiber optic. High speed imaging is enabled by on/off times and wavelength change times on the order of tens of microseconds. Laser lines can be custom selected and combined to meet any research need and the modular design readily allows for future expansion.

3i LaserStack with Fiber Switcher
Available lasers

 

Highest quality single mode optical fibers allow coupling of any combination of the following wavelengths to the microscope:

 

LaserStack 405nm – 50mW, 100mW

LaserStack 445nm – 75mW

LaserStack 473nm – 75mW

LaserStack 488nm – 20mW, 50mW, 150mW

LaserStack 514nm – 4omW, 100mW

LaserStack 561nm – 20mW, 50mW, 150mW

LaserStack 594nm – 60mW, 100mW

LaserStack 640nm – 100mW

LaserStack 660nm – 40mW, 100mW, 140mW

Additionally, the following stand-alone laser products can be individually coupled to the microscope:

 

SingleLine 405nm 100mW

SingleLine 445nm 75mW

SingleLine 488nm 50mW and 150mW

SingleLine 640nm 100mW

SingleLine 730nm 30mW

SingleLine 785nm 100mW

Rapid switching between devices

 

The optional LaserStack FiberSwitcher™ allows sub-millisecond changing between up to four illuminating devices via a high speed galvo-driven mirror. FiberSwitcher can rapidly toggle laser output between such devices as:

Spinning Disk Confocal – CSU-X1 or the new CSU-W1

TIRF – motorized or manual illuminators for Total Internal Reflection Fluorescence microscopy

Vector™ – high speed point scanner for FRAP and other photomanipulation techniques

Phasor™ – SLM based digital holography illuminator for photostimulation and other photomanipulation techniques

FLIM – frequency based fluorescence lifetime imaging for quantitative fluorescence measurements

 

Pockels Cell

 

LaserStack can integrate a Pockels cell for frequency based FLIM illumination in combination with a modulated intensifier detector and the SlideBook FLIM module.

 

LaserStream™

 

LaserStream can change the output wavelength from LaserStack with each camera exposure. Users can program LaserStream to select laser illumination in any combination or pattern they choose, limited in speed only by the exposure time of the camera.

 

DeMuxer™

 

Designed to allow separation of individual laser lines from the output fiber of LaserStack, DeMuxer allows single wavelengths to be channeled to separate devices or to separate inputs on a single device such as the Olympus cell^TIRF™ system.

3i FiberSwitcher detail

mSwitcher

The 3i mSwitcher high speed galvo port switcher allows rapid, 1ms selection of three optical output (or three input) paths for one input (output). This enables direct combination of multiple devices and methods.

mSwitcher PS4

mSwitcher PS3

mSAC mounted to the
mSwitcher PS4

mSAC mounted to the
mSwitcher PS3

mSAC

Spherical aberration (SA) is the most serious monochromatic defect that occurs with microscope objectives. Indeed, SA is the limiting physics for most of 3-D microscopy. SA causes the image to appear blurry with less contrast. SA is generated when there is material with a different refractive index than the lens designer’s specification between the front of the objective and the image plane. Typically, SA becomes more serious as one focuses deeper into samples.

mSAC Data with Confocal
Living samples (GFP Histones) imaged with a 63x 1.4 NA oil objective, SAC technology and a spinning disk confocal: Neutral SAC (left) and Rolling SAC (right).
GFP Mouse Embryo 100µm, imaging distance, 40x objective with Neutral SAC (left) and Rolling SAC (right).

TTL Sync

TTL Synchronization (TTL Sync) provides sophisticated electronic control of carefully timed devices in a microscopy system. In combination with SlideBook software, TTL Sync can both create and receive triggering signals from a variety of devices and orchestrate their cooperative function throughout an experiment. Timing that is simply not achievable through standard USB and serial connection to a PC is made possible by custom circuitry operating independently of but in cooperation with the computer and operating system.

 

At a basic level, TTL Sync provides software control of analog/digital I/O boards, utilizing them to direct a variety of signals via TTL and other methods. Key to this functionality is pulse generation and recording. This module can also be used to synchronize external stimulation and data collection instruments, such as electrodes, patch clamp recording devices, and perfusion systems.

Connector-side of
TTL Synchronization with
LaserStack input.
Connector-side of the small
Synchronization module.

VectorTIRF

Motorized Spinning X,Y TIRF Illumination

  • TIRF illuminator allowing high-speed continuous 360° illumination
  • Switch between single point illumination and spinning illumination
  • Independently set spin angles and durations
  • Millisecond TIRF angle switch time
  • Illuminate with multiple wavelengths with millisecond switching
  • Combine with spinning disc, FRAP and/or FLIM in a single multimodal system
Specifications
Laser Lines 405nm, 445nm, 488nm, 515nm, 561nm, 594nm, 640nm
Laser Power Output >20 mW at the back aperture
Compatibility Mounts to the sideport on Zeiss, Olympus, Nikon and Leica microscopes

Supported biphasic lipid bilayers (6nm thick) with fixed-position TIRF illumination (left) and VectorTIRF motorized spinning TIRF illumination with 5ms rotation speed and 20ms exposure (right).

Images courtesy Dr. Emmanuel Derivery (University of Geneva, Dr. Gonzalez-Gaitan lab) and Dr. Nicolas Chiaruttini (University of Geneva, Dr. Roux lab)
Fixed position TIRF
VectorTIRF
Motorized Spinning X,Y TIRF Illumination

Bifurcator

Bifurcator™ can be both an optical splitter and a signal combiner, providing spectral separation or combination in a perfect image relay.

 

An exchangeable dichromatic mirror, precision alignment of each port, and the ability to combine 1.0x and 1.2x magnifications makes this a practical and powerful imaging tool.

Optimizer

Optimizer™ creates a perfect image relay for optimal positioning of emission filter wheels in a collimated infinity space.

 

Magnification can be chosen as either 1.0x or 1.2x to match various detectors.

DeMuxer

The DeMuxer allows separation of the individual laser lines from a LaserStack laser launch. Individual signals can then be sent to any device requiring a single wavelength such as the inputs on an Olympus cell^TIRF™ system.

Let’s work together






SALES

Phone: +1 (303)-607-9429 x1
Email: sales@intelligent-imaging.com

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SUPPORT

Phone: +1 (303)-607-9429 x2
Email: support@intelligent-imaging.com

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