Cleared Tissue LightSheet (CTLS) is a large field microscope designed to image whole organs at high speed. CTLS creates a focused sheet with a narrow waist for better optical sectioning, then uses a spatial light modulator (SLM) to rapidly shift the waist of the sheet along the axis of propagation. A dual excitation setup allows imaging from the right and left sides of the specimen for optimal lightsheet projection throughout. Piezoelectric stages move the specimen in x, y, and z with sub-micron resolution. The result is clear: a 1 cm3 volume can be imaged at up to 1µm x 1µm x 3µm (XYZ) resolution, and a cleared mouse brain can be imaged in as little as 1.5 hours.
Lightsheet microscopy is a powerful technique for imaging large specimens by taking full advantage of emerging tissue clearing methods. The chemistry behind these techniques has advanced to where we can easily penetrate 1, 5 even 10mm into a specimen with a focused sheet of light. In combination with a macro zoom microscope using high NA large field of view lenses, Cleared Tissue LightSheet can image large field sizes with high resolution in short periods of time.
The optical sectioning ability of a light sheet is dependent on the waist of the focused beam, and the waist of the beam is inversely proportional to its length. Imaging large, cleared specimens typically requires long beams with a thick waist producing poor optical sectioning. To dramatically improve optical sectioning, CTLS uses an SLM programmed by SlideBook software to create short beams with narrow waists that are rapidly translated across the field of view. The resulting data has an excellent axial resolution compared to a non-translated focused beam.
Allows for optimal sheet penetration across wide specimens and avoidance of opaque structures that may be present on one side but not the other
Macro lenses at 1x/0.25NA and 1.5x/0.37NA can image through any cleared organ with excellent resolution
Sub-micron resolution stages allow precise positioning of the specimen in sample chambers sized to fit the biology
Fiber-coupled laser combiner allows up to six lasers covering the entire visible spectrum at multiple power levels
SlideBook directs all hardware synchronization and data capture, creating 3D datasets at over 1TB ready for analysis and rendering
Available DDN® unified storage systems allow direct acquisition and analysis without time-consuming file transfers for 200TB to over 1PB
1 µm x 1 µm x 3 µm
< 1 min/mm3
25mm x 25mm x 25mm (XYZ)
Multiple glass specimen chambers optimized for 1x/0.25NA and 1.5x/0.37NA objectives, with specimen holder. Custom-shaped specimen holders available upon request.
Organic or aqueous clearing solutions
2048×2048 16-bit sCMOS
LaserStack compact modular laser launch with 488nm and 561nm lasers standard
Up to 4 additional wavelengths upon request
Piezoelectric with sub-micron resolution in X, Y and Z
SlideBook™ software for acquisition and GPU-accelerated analysis